Plant Genetics, The Weizmann Institute of Science, 76 100, Rehovot, Israel.
Theor Appl Genet. 1985 Mar;69(5-6):583-9. doi: 10.1007/BF00251108.
Total endosperm proteins extracted from both several common wheat cultivars and some intervarietal substitution lines derived from them were fractionated according to their molecular weight in a high resolution one-dimensional gel electrophoresis. The four donor cultivars and the recipient one - 'Chinese Spring', possessed differentially migrating protein bands in the fractions of high molecular weight (HMW) glutenins and gliadins. Several of these bands were identified for the first time in this study. By utilizing intervarietal substitution lines the control of the HMW glutenins and gliadins by chromosomes of homoeologous group 1 was either reaffirmed or, for the new bands, established. Several HMW gliadin subunits showed a considerable variation in their staining intensity in the intervarietal substitution lines indicating that their expression was dependent on the genetic background.
从几种普通小麦品种和由它们衍生的一些品种间代换系中提取的总胚乳蛋白，根据其分子量在高分辨率一维凝胶电泳中进行了分级。四个供体品种和受体品种“中国春”在高分子量（HMW）谷蛋白和醇溶蛋白的馏分中具有不同迁移的蛋白带。其中一些条带是本研究中首次鉴定的。利用品种间代换系，同源群 1 染色体对 HMW 谷蛋白和醇溶蛋白的控制得到了再次确认，或者对于新的条带，也得到了确认。几种 HMW 醇溶蛋白亚基在品种间代换系中的染色强度有相当大的变化，表明它们的表达依赖于遗传背景。
