Department of Agronomy, University of Illinois, 61801, Urbana, IL, USA.
Plant Cell Rep. 1985 Dec;4(6):304-6. doi: 10.1007/BF00269884.
Suspension cultured Nicotiana tabacum (tobacco) cells grow slowly on intermediates of the purine degradation pathway (hypoxanthine, xanthine, uric acid, allantoin, and urea) as their sole nitrogen source indicating that this degradation pathway is operative in these cells. The hypoxanthine analog, allopurinol inhibited tobacco cell growth on hypoxanthine but not uric acid. This helps confirm that the site of action of allopurinol is the conversion of hypoxanthine to uric acid by xanthine oxidase. Attempts to select cells which could grow in the presence of allopurinol with hypoxanthine as the nitrogen source were not successful.
悬浮培养的烟草细胞（烟草）以嘌呤降解途径的中间产物（次黄嘌呤、黄嘌呤、尿酸、尿囊素和尿素）作为唯一氮源缓慢生长，这表明该降解途径在这些细胞中是有效的。次黄嘌呤类似物别嘌呤醇抑制烟草细胞在次黄嘌呤上的生长，但不抑制尿酸上的生长。这有助于证实别嘌呤醇的作用部位是黄嘌呤氧化酶将次黄嘌呤转化为尿酸。尝试选择能够在含有别嘌呤醇和次黄嘌呤作为氮源的情况下生长的细胞的尝试没有成功。
