Young J D, Cohn Z A, Podack E R
Science. 1986 Jul 11;233(4760):184-90. doi: 10.1126/science.2425429.
The ninth component of complement (C9) and the pore-forming protein (PFP or perforin) from cytotoxic T lymphocytes polymerize to tubular lesions having an internal diameter of 100 A and 160 A, respectively, when bound to lipid bilayers. Polymerized C9, assembled by slow spontaneous or rapid Zn2+-induced polymerization, and polyperforin, which is assembled only in the presence of Ca2+, constitute large aqueous pores that are stable, nonselective for solutes, and insensitive to changes of membrane potential. Monospecific polyclonal antibodies to purified C9 and PFP show cross-reactivity, suggesting structural homology between the two molecules. The structural and functional homologies between these two killer molecules imply an active role for pore formation during cell lysis.
补体的第九成分（C9）和细胞毒性T淋巴细胞的成孔蛋白（PFP或穿孔素）与脂质双层结合时，分别聚合成内径为100埃和160埃的管状损伤。通过缓慢自发或快速锌离子诱导聚合组装的聚合C9，以及仅在钙离子存在下组装的多聚穿孔素，构成了大的水通道，这些通道稳定、对溶质无选择性且对膜电位变化不敏感。针对纯化的C9和PFP的单特异性多克隆抗体表现出交叉反应性，表明这两种分子之间存在结构同源性。这两种杀伤分子之间的结构和功能同源性意味着在细胞裂解过程中孔形成发挥着积极作用。
