Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, U.S.A.
Biosci Rep. 2023 Oct 31;43(10). doi: 10.1042/BSR20231265.
Many proinflammatory proteins are released via the necrotic form of cell death known as pyroptosis. Sometimes known as gasdermin D (GSDMD) dependent cell death, pyroptosis results from the formation of pores in the plasma membrane leading to eventual cell lysis. Seeking to understand the magnitude of this cell lysis we measured the size of proteins released during pyroptosis. We demonstrate that there is no restriction on the size of soluble proteins released during pyroptosis even at early timepoints. However, even though large molecules can exit the dying cell, organelles are retained within it. This observation indicates that complete cell rupture may not be a consequence of pyroptosis, and that plasma membrane architecture is retained.
许多促炎蛋白通过称为细胞焦亡的细胞坏死形式释放。细胞焦亡有时也被称为gasdermin D（GSDMD）依赖性细胞死亡，是由于质膜形成孔道导致最终细胞裂解而产生的。为了了解这种细胞裂解的程度，我们测量了细胞焦亡过程中释放的蛋白质的大小。我们证明，即使在早期时间点，细胞焦亡过程中释放的可溶性蛋白质的大小也没有限制。然而，尽管大分子可以离开垂死的细胞，但细胞器仍保留在其中。这一观察结果表明，完全的细胞破裂可能不是细胞焦亡的结果，而且质膜的结构得以保留。
