玻璃化冷冻后人类原始卵泡体外向窦前卵泡发育。
In vitro development of human primordial follicles to preantral stage after vitrification.
机构信息
Department of Biomedical and Molecular Sciences, Faculty of Health Sciences, Queen's University, Kingston, Ontario, Canada, K7L 3N6.
出版信息
J Assist Reprod Genet. 2013 Nov;30(11):1397-406. doi: 10.1007/s10815-013-0105-z. Epub 2013 Oct 25.
Abstract
PURPOSE
The aim was to culture primordial follicles in vitro to reach preantral stage in vitrified human ovarian tissue.
METHODS
Ovarian tissue samples were obtained from six women. Tissue strips were vitrified by infiltration with a cryoprotectant followed by mounting on a stainless steel carrier. After culturing for 7 days the morphology and developmental stages of follicles enclosed in fresh and vitrified groups were analyzed.
RESULTS
High proportion of viable follicles in vitrified ovarian strips was obtained. After culturing for 7 days the percentage of secondary and preantral follicles increased significantly (P < 0.05) whereas primordial and transitory follicles showed a significant decrease (P < 0.05) compared to their respective counterparts at day 0 of culture.
CONCLUSIONS
Vitrification of ovarian strips with an improved carrier device and culturing of follicles in ovarian strips after warming yielded developed follicles with high viability and morphological integrity that may be suitable for use in fertility preservation among cancer patients.
摘要
目的
本研究旨在通过将原始卵泡体外培养至玻璃化人卵巢组织的腔前卵泡阶段，来实现这一目标。
方法
本研究从 6 名女性中获取了卵巢组织样本。组织条带通过渗透用冷冻保护剂进行玻璃化，然后装在不锈钢载体上。在培养 7 天后，分析新鲜和玻璃化组中封闭的卵泡的形态和发育阶段。
结果
获得了高比例的玻璃化卵巢条带中有活力的卵泡。培养 7 天后，与培养第 0 天相比，次级和腔前卵泡的百分比显著增加（P<0.05），而原始卵泡和过渡卵泡的数量显著减少（P<0.05）。
结论
使用改进的载体装置对卵巢条带进行玻璃化处理，并在解冻后对卵泡进行培养，可获得具有高活力和形态完整性的发育卵泡，这可能适合用于癌症患者的生育力保存。
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