Marek LeEtta J, Koskinen William C
Minnesota Department of Agriculture, St Paul, MN, USA.
Pest Manag Sci. 2014 Jul;70(7):1158-64. doi: 10.1002/ps.3684. Epub 2013 Dec 26.
There is a need for a simple, fast, efficient and sensitive method for analysis of glyphosate and its degradate aminomethylphosphonic acid (AMPA) in diverse matrices such as water, vegetation and soil.
Aqueous extracts from water, vegetation and soil were passed through reverse-phase and cation-exchange columns and directly injected into a tandem mass spectrometer using only a guard column for separation. Extraction efficiencies from the three matrices were >80% for both glyphosate and AMPA. The method reporting levels (MRLs) for glyphosate in water, vegetation and soil were 3.04 µg L(-1) , 0.05 mg kg(-1) and 0.37 mg kg(-1) respectively. AMPA MRLs were 5.06 µg L(-1) for water, 0.08 mg kg(-1) for vegetation and 0.61 mg kg(-1) for soil.
A validated, simple and efficient liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for routine analysis of glyphosate and AMPA in water, vegetation and soil that uses minimal sample handling and clean-up will facilitate the additional environmental research needed to address the continuing concerns related to increasing glyphosate use.
需要一种简单、快速、高效且灵敏的方法，用于分析水、植被和土壤等多种基质中的草甘膦及其降解产物氨甲基膦酸（AMPA）。
水、植被和土壤的水提取物通过反相柱和阳离子交换柱，仅使用保护柱进行分离后直接注入串联质谱仪。草甘膦和AMPA从这三种基质中的提取效率均>80%。水、植被和土壤中草甘膦的方法报告水平（MRL）分别为3.04 μg L⁻¹、0.05 mg kg⁻¹和0.37 mg kg⁻¹。AMPA的MRL，水中为5.06 μg L⁻¹，植被中为0.08 mg kg⁻¹，土壤中为0.61 mg kg⁻¹。
一种经过验证的、简单高效的液相色谱 - 串联质谱（LC-MS/MS）方法，用于水、植被和土壤中草甘膦和AMPA的常规分析，该方法只需最少的样品处理和净化步骤，将有助于开展更多环境研究，以解决因草甘膦使用增加而持续引发的问题。
