USDA, ARS, HNRC on Aging at Tufts University, 711 Washington St., 02111, Boston, MA.
Biol Trace Elem Res. 1987 Apr;12(1):335-50. doi: 10.1007/BF02796690.
Methods for the measurement of the zinc content of blood components (plasma, erythrocytes, platelets, mononuclear leukocytes, and polymorphonuclear neutrophils) as indicators of the zinc status of animals have been reviewed and evaluated. The values of plasma or serum zinc concentrations as indicators of zinc status in experimental animals or humans is questionable. Consequently, the zinc content of blood cellular components has been suggested as better indicators of zinc status. Methodological problems, such as incomplete cellular separation or zinc contamination, occur in some of the procedures applied to the quantitation of blood cellular component zinc. Specialized microprobe procedures involving, for example, proton-induced X-ray fluorescence, scanning ion technology, mass spectrometery, or laser microprobe mass analysis might be used to measure the absolute zinc level in individual cells subjected to minimal handling. Then, more conventional methods can be tested against the true standard. Nevertheless, there appears to be species differences in regard to the response of blood cellular zinc concentrations to dietary zinc deficiency. Blood cellular component zinc is conserved during severe zinc deficiency in the rat. In contrast, the zinc content of blood cellular components in humans may reflect the whole body zinc status.
已经对血液成分（血浆、红细胞、血小板、单核白细胞和多形核白细胞）中锌含量的测量方法进行了综述和评估，这些方法可作为动物锌状况的指标。作为实验动物或人类锌状况指标的血浆或血清锌浓度值是有问题的。因此，血液细胞成分的锌含量被认为是更好的锌状况指标。在应用于血液细胞成分锌定量的一些程序中，会出现细胞分离不完全或锌污染等方法学问题。涉及质子诱导 X 射线荧光、扫描离子技术、质谱或激光微探针质谱分析等特殊的微探针程序可以用于测量经过最小处理的单个细胞的绝对锌水平。然后，可以针对真实标准对更常规的方法进行测试。然而，在血液细胞锌浓度对饮食锌缺乏的反应方面似乎存在物种差异。在大鼠严重缺锌时，血液细胞成分的锌会被保存下来。相比之下，人类血液细胞成分的锌含量可能反映全身的锌状况。
