Milne D B, Ralston N V, Wallwork J C
J Nutr. 1985 Aug;115(8):1073-8. doi: 10.1093/jn/115.8.1073.
Several investigators have suggested that the measurement of leukocyte zinc may be useful for the assessment of zinc nutriture. However, in an earlier study, we found that the methods used did not adequately separate platelets from the different leukocyte populations. We subsequently developed a method which does adequately separate platelets, mononucleated cells (MNC), polymorphonucleated cells (PMN), and erythrocytes (RBC) from a single blood sample. This method was applied to a study of severe zinc deficiency in adult male rats. Significant reductions in plasma and femur zinc indicated severe zinc deficiency in the zinc-deficient animals compared with pair-fed or ad libitum-fed control rats in two separate experiments. However, we noted no differences in the zinc content of the MNC, PMN, RBC or lymph node lymphocytes, on a per cell basis, between the zinc-deficient and ad libitum- or pair-fed control groups of rats. A minor, but not significant (P greater than 0.05) reduction of platelet zinc was noted in zinc-deficient rats in two separate studies. These data indicate that the zinc contents of blood cellular components are not sensitive indicators of zinc deficiency in rats.
几位研究者提出，测量白细胞锌含量可能有助于评估锌营养状况。然而，在早期的一项研究中，我们发现所使用的方法不能充分地将血小板与不同的白细胞群体分离。随后，我们开发了一种方法，该方法能够从单一血液样本中充分分离出血小板、单核细胞（MNC）、多形核细胞（PMN）和红细胞（RBC）。此方法应用于成年雄性大鼠严重锌缺乏的研究。在两项独立实验中，与配对喂养或自由进食的对照大鼠相比，血浆和股骨锌含量显著降低表明缺锌动物存在严重锌缺乏。然而，我们注意到，在缺锌大鼠与自由进食或配对喂养的对照大鼠组之间，单个细胞的MNC、PMN、RBC或淋巴结淋巴细胞的锌含量并无差异。在两项独立研究中，缺锌大鼠的血小板锌含量有轻微降低，但不显著（P大于0.05）。这些数据表明，血细胞成分中的锌含量不是大鼠锌缺乏的敏感指标。
