National Engineering Laboratory for AIDS Vaccine, College of Life Science, Jilin University, Changchun, Jilin, China.
J Pept Sci. 2014 Jan;20(1):46-54. doi: 10.1002/psc.2587. Epub 2013 Nov 19.
Efficient gene transfer is a critical goal in retroviral transduction. Several peptides capable of forming amyloid fibrils, such as the 39-residue semen-derived infection-enhancing peptide (SEVI), have demonstrated the ability to boost retroviral gene delivery. Here, a 13-residue peptide P13 (Ac-(671) NWFDITNWLWYIK(683)) derived from the membrane-proximal external region of the human immunodeficiency virus type 1 (HIV-1) gp41 transmembrane protein, together with its 16-residue peptide derivative (P16) were found to enhance HIV-1 infection significantly. Both peptides, P13 and P16, could form amyloid fibril structures to potently enhance HIV-1 infectivity. Further investigations showed that both aromatic Trp residues and cationic Lys residues contributed to the enhancement of HIV-1 infection by these two active peptides. P16 could more effectively augment HIV-1 YU-2 infection than SEVI, implying its potential applications as a tool in the lab to improve gene transfer rates.
高效的基因转移是逆转录病毒转导的一个关键目标。一些能够形成淀粉样纤维的肽，如 39 个残基的精液衍生的感染增强肽（SEVI），已经证明能够增强逆转录病毒基因传递。在这里，一种来自人类免疫缺陷病毒 1 型（HIV-1）gp41 跨膜蛋白膜近端外部区域的 13 个残基肽 P13（Ac-（671）NWFDITNWLWYIK（683））与其 16 个残基肽衍生物（P16）一起被发现能够显著增强 HIV-1 感染。这两种肽，P13 和 P16，都可以形成淀粉样纤维结构，从而有效地增强 HIV-1 的感染力。进一步的研究表明，这两种活性肽的芳香色氨酸残基和阳离子赖氨酸残基都有助于增强 HIV-1 的感染。P16 比 SEVI 更有效地增强 HIV-1 YU-2 的感染，这意味着它有可能作为一种工具在实验室中提高基因转移率。
