Department of Biology, Southern Arkansas University, 71753, Magnolia, Arkansas.
J Chem Ecol. 1992 Feb;18(2):197-207. doi: 10.1007/BF00993753.
The aim of this investigation was to determine if sorgoleone (SGL), a hydrophobic compound inSorghum bicolor (L.) Moench root exudate, interferes with mitochondrial functions. Tests were conducted on mitochondria isolated from etiolated soybean [Glycine max (L.) Merr.] and corn (Zea mays L.) seedlings. The data show SGL is a potent inhibitor of state 3 and state 4 respiration rates in both soybean and corn. Using either NADH, succinate, or malate as substrate, the I50 was about 0.5μM SGL for state 3 and 5.0μM for state 4 based on 0.3-0.5 mg mitochondrial protein. Absorption spectra indicate SGL blocks electron transport at theb-c 1 complex. These data show that disruption of mitochondrial function may be a mechanism of SGL-mediated growth inhibition previously reported and demonstrate a probable role of SGL inSorghum allelopathy.
本研究旨在确定高粱根渗出物中的疏水性化合物 sorgoleone（SGL）是否会干扰线粒体功能。在黄化大豆[ Glycine max（L.）Merr.]和玉米（ Zea mays L.）幼苗分离的线粒体上进行了测试。数据表明，SGL 是大豆和玉米中状态 3 和状态 4 呼吸速率的有效抑制剂。使用 NADH、琥珀酸或苹果酸作为底物，基于 0.3-0.5mg 线粒体蛋白，I50 约为 0.5μM SGL 用于状态 3，5.0μM 用于状态 4。吸收光谱表明 SGL 在线粒体 b-c1 复合物处阻断电子传递。这些数据表明，线粒体功能的破坏可能是先前报道的 SGL 介导的生长抑制的一种机制，并证明了 SGL 在高粱化感作用中的可能作用。
