The Reproduction Laboratory of Traditional Chinese Medicine and Western Medicine, The Second Affiliated Clinical Medical School, Chengdu University of Traditional Chinese Medicine, Chengdu, 610041, Sichuan, China.
Hum Cell. 2014 Jan;27(1):5-11. doi: 10.1007/s13577-013-0084-5. Epub 2013 Nov 20.
This study investigated the effects of the compound oleanolic acid (OA) in inducing mouse embryonic stem cells (MESC) to differentiate towards germ cells (GC). MESC 1B10 was used as the model cell. 1B10 was cultured, and embryoid bodies (EBs) were produced from 1B10. The EBs were allowed to attach to the bottoms of culturing disks and grow. OA was added into the medium to induce the EBs to differentiate. Retinoic acid (RA) was used as the positive drug. After 72 h, total RNA was extracted, cDNA was synthesized, and real-time fluorescence quantitative PCR was performed to measure the transcriptional expression profiles of 11 reproduction-related genes affected by OA and RA, respectively. When the data were compared, it was found that OA up-regulated the transcriptional levels of Oct-4, GDF-9, Stra8, Mvh, ZP2, ZP3, Itga6, and TP2, and down-regulated transcriptional levels of SCP3, ZP1, and Itgb1; RA up-regulated the transcriptional levels of GDF-9, Stra8, Mvh, ZP2, ZP3, Itga6, Itgb1, and TP2, and down-regulated the transcriptional levels of Oct-4, SCP3, and ZP1. The data showed that OA and RA had similar effects in inducing differentiation of MESC towards GC.
本研究探讨了化合物齐墩果酸（OA）诱导小鼠胚胎干细胞（MESC）向生殖细胞（GC）分化的作用。以 1B10 细胞作为模型细胞。培养 1B10 细胞，使其形成类胚体（EB）。将 EB 贴附在培养皿底部并使其生长。向培养基中加入 OA 诱导 EB 分化。视黄酸（RA）用作阳性药物。72 小时后，提取总 RNA，合成 cDNA，并进行实时荧光定量 PCR，分别测量 OA 和 RA 影响的 11 个生殖相关基因的转录表达谱。比较数据后发现，OA 上调了 Oct-4、GDF-9、Stra8、Mvh、ZP2、ZP3、Itga6 和 TP2 的转录水平，下调了 SCP3、ZP1 和 Itgb1 的转录水平；RA 上调了 GDF-9、Stra8、Mvh、ZP2、ZP3、Itga6、Itgb1 和 TP2 的转录水平，下调了 Oct-4、SCP3 和 ZP1 的转录水平。数据表明，OA 和 RA 在诱导 MESC 向 GC 分化方面具有相似的作用。
