Kašička Václav
Institute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Prague, Czech Republic.
Electrophoresis. 2018 Jan;39(1):209-234. doi: 10.1002/elps.201700295. Epub 2017 Sep 25.
The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.
本综述全面概述了2015年、2016年以及截至2017年年中左右高性能毛细管和微芯片电分离方法（区带电泳、等速电泳、等电聚焦、亲和电泳、电动色谱和电色谱）在肽的分析、微尺度分离、纯化以及物理化学和生化表征方面的最新进展和应用。描述了肽的电迁移性质研究及其分析方法（样品预分离、预浓缩和衍生化、吸附抑制和电渗流控制以及检测）的进展。介绍了特定毛细管电泳和毛细管电色谱方法的新进展，并报道了它们在肽分析中的几种应用类型：定性和定量分析、复杂（生物）基质中的测定、化学和酶促反应以及物理变化的监测、氨基酸、序列和手性分析以及蛋白质的肽图谱分析。展示了一些微制备肽分离，并证明了毛细管电泳和毛细管电色谱方法提供肽重要物理化学特性的能力。
