Institute for the Application of Nuclear Energy-INEP, Department for Endocrinology and Immunoradiology, Belgrade, Serbia.
Otolaryngol Head Neck Surg. 2014 Feb;150(2):201-9. doi: 10.1177/0194599813512781. Epub 2013 Nov 19.
We aimed to investigate the role of caveolin-1 in papillary thyroid carcinoma pathogenesis.
Case series with chart review.
Institute for the Application of Nuclear Energy.
We evaluated the expression of caveolin-1 in papillary thyroid carcinoma (PTC) by Western blot (WB) and compared the findings with immunohistochemical (IHC) expression of both epithelial and stromal caveolin-1 on the corresponding histological specimens. The results were related to clinicopathological features and BRAF mutation status.
Caveolin-1 expression was found in malignant thyroid epithelium and more abundantly in tumor stroma but varied in both compartments within and between PTC subtypes. Caveolin-1 expression in the epithelium was more intense in classical PTC than in the other histological types. On the contrary, stromal caveolin-1 expression was stronger in the follicular, solid, and trabecular PTC variants than in classical PTC. Trends for down-regulation of caveolin-1 expression in epithelium and up-regulation in stroma from the classical via follicular to the solid variant were observed. The relation of WB and IHC results with clinicopathological parameters showed lower caveolin-1 tissue content in BRAF mutated tumors (P < .05), a positive correlation of epithelial caveolin-1 expression with lymph node metastasis (P < .05), and a negative association of stromal caveolin-1 expression with the degree of neoplastic infiltration and BRAF status.
Altered expression of caveolin-1 in the thyroid epithelial and stromal compartments may be involved in the pathogenesis of PTC. The potential clinical significance of caveolin-1 expression, as well as its relation to BRAF mutation status, deserves further investigation.
研究小窝蛋白-1 在甲状腺乳头状癌发病机制中的作用。
病例系列及图表回顾。
应用核能源研究所。
我们通过 Western blot（WB）评估了甲状腺乳头状癌（PTC）中 caveolin-1 的表达，并将结果与相应组织学标本上皮和基质 caveolin-1 的免疫组织化学（IHC）表达进行比较。结果与临床病理特征和 BRAF 突变状态相关。
在恶性甲状腺上皮细胞和肿瘤基质中发现 caveolin-1 表达，但在 PTC 亚型内和之间的两个隔室中均存在差异。经典型 PTC 中上皮 caveolin-1 表达比其他组织学类型更强烈。相反，滤泡状、实体状和小梁状 PTC 变体中基质 caveolin-1 表达更强。观察到从经典型到滤泡状再到实体状变体，上皮 caveolin-1 表达下调和基质 caveolin-1 表达上调的趋势。WB 和 IHC 结果与临床病理参数的关系表明，BRAF 突变肿瘤中的 caveolin-1 组织含量较低（P <.05），上皮 caveolin-1 表达与淋巴结转移呈正相关（P <.05），基质 caveolin-1 表达与肿瘤浸润程度和 BRAF 状态呈负相关。
甲状腺上皮和基质隔室中小窝蛋白-1 的表达改变可能与 PTC 的发病机制有关。caveolin-1 表达的潜在临床意义及其与 BRAF 突变状态的关系值得进一步研究。
