Liu Yu, Liang Yanan, Li Ming, Liu Duanyang, Tang Jing, Yang Weiwei, Tong Dandan, Jin Xiaoming
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
Oncol Lett. 2018 Oct;16(4):4263-4270. doi: 10.3892/ol.2018.9200. Epub 2018 Jul 24.
Papillary thyroid cancer (PTC) is the most common type of thyroid malignancy, and it is often observed to overexpress epidermal growth factor receptor (EGFR). Previous research has indicated that EH domain-containing 1 (EHD1) is associated with EGFR-mediated endocytotic recycling in multiple tumor types. The objective of the present study was to determine the protein expression levels and clinical significance of EHD1, EGFR, caveolin-1 (CAV-1) and RAB11 family interacting protein 3 (RAB11FIP3) in PTC. PTC specimens were analyzed for EHD1, EGFR, CAV-1 and RAB11FIP3 expression via immunohistochemistry and western blotting. The associations between protein expression and clinicopathological features were assessed. EHD1, EGFR, CAV-1 and RAB11FIP3 expression levels were increased in human PTC. Additionally, the expression level of EHD1 protein was significantly associated with tumor size, lymph node metastasis and EGFR expression (P<0.05). CAV-1 was associated with tumor size and EGFR expression (P<0.05). EGFR was only associated with lymph node metastasis (P=0.027) and RAB11FIP3 was not associated with any clinicopathological characteristics. The correlations between EHD1 and EGFR (r=0.564, P<0.05), CAV-1 (r=0.865, P<0.01) and RAB11FIP3 (r=0.504, P<0.05) were statistically significant. Overall, EHD1, CAV-1 and RAB11FIP3, which are key proteins in endocytotic recycling, promote PTC tumorigenesis through the regulation of the transport of EGFR.
乳头状甲状腺癌（PTC）是最常见的甲状腺恶性肿瘤类型，常观察到其表皮生长因子受体（EGFR）过表达。先前的研究表明，含EH结构域蛋白1（EHD1）在多种肿瘤类型中与EGFR介导的内吞再循环相关。本研究的目的是确定EHD1、EGFR、小窝蛋白-1（CAV-1）和RAB11家族相互作用蛋白3（RAB11FIP3）在PTC中的蛋白表达水平及临床意义。通过免疫组织化学和蛋白质印迹法分析PTC标本中EHD1、EGFR、CAV-1和RAB11FIP3的表达情况。评估蛋白表达与临床病理特征之间的关联。人PTC中EHD1、EGFR、CAV-1和RAB11FIP3的表达水平升高。此外，EHD1蛋白表达水平与肿瘤大小、淋巴结转移及EGFR表达显著相关（P<0.05）。CAV-1与肿瘤大小和EGFR表达相关（P<0.05）。EGFR仅与淋巴结转移相关（P=0.027），而RAB11FIP3与任何临床病理特征均无关联。EHD1与EGFR（r=0.564，P<0.05）、CAV-1（r=0.865，P<0.01）和RAB11FIP3（r=0.504，P<0.05）之间的相关性具有统计学意义。总体而言，作为内吞再循环关键蛋白的EHD1、CAV-1和RAB11FIP3通过调节EGFR的转运促进PTC的肿瘤发生。
