Kim Young, Choi Yoo Duk, Choi Chan, Nam Jong-Hee
Department of Pathology, Chonnam National University Medical School, Gwangju, Korea.
Korean J Pathol. 2013 Oct;47(5):458-65. doi: 10.4132/KoreanJPathol.2013.47.5.458. Epub 2013 Oct 25.
A clonality test for immunoglobulin (IG) and T cell receptor (TCR) is a useful adjunctive method for the diagnosis of lymphoproliferative diseases (LPDs). Recently, the BIOMED-2 multiplex polymerase chain reaction (PCR) assay has been established as a standard method for assessing the clonality of LPDs. We tested clonality in LPDs in Koreans using the BIOMED-2 multiplex PCR and compared the results with those obtained in European, Taiwanese, and Thai participants. We also evaluated the usefulness of the test as an ancillary method for diagnosing LPDs.
Two hundred and nineteen specimens embedded in paraffin, including 78 B cell lymphomas, 80 T cell lymphomas and 61 cases of reactive lymphadenitis, were used for the clonality test.
Mature B cell malignancies showed 95.7% clonality for IG, 2.9% co-existing clonality, and 4.3% polyclonality. Mature T cell malignancies exhibited 83.8% clonality for TCR, 8.1% co-existing clonality, and 16.2% polyclonality. Reactive lymphadenitis showed 93.4% polyclonality for IG and TCR. The majority of our results were similar to those obtained in Europeans. However, the clonality for IGK of B cell malignancies and TCRG of T cell malignancies was lower in Koreans than Europeans.
The BIOMED-2 multiplex PCR assay was a useful adjunctive method for diagnosing LPDs.
免疫球蛋白（IG）和T细胞受体（TCR）的克隆性检测是诊断淋巴增殖性疾病（LPD）的一种有用的辅助方法。最近，BIOMED-2多重聚合酶链反应（PCR）检测已被确立为评估LPD克隆性的标准方法。我们使用BIOMED-2多重PCR检测了韩国LPD患者的克隆性，并将结果与欧洲、台湾和泰国参与者的结果进行了比较。我们还评估了该检测作为诊断LPD辅助方法的实用性。
使用219例石蜡包埋标本进行克隆性检测，其中包括78例B细胞淋巴瘤、80例T细胞淋巴瘤和61例反应性淋巴结炎。
成熟B细胞恶性肿瘤的IG克隆性为95.7%，共存克隆性为2.9%，多克隆性为4.3%。成熟T细胞恶性肿瘤的TCR克隆性为83.8%，共存克隆性为8.1%，多克隆性为16.2%。反应性淋巴结炎的IG和TCR多克隆性为93.4%。我们的大多数结果与欧洲人获得的结果相似。然而，韩国人B细胞恶性肿瘤的IGK和T细胞恶性肿瘤的TCRG克隆性低于欧洲人。
BIOMED-2多重PCR检测是诊断LPD的一种有用的辅助方法。
