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监测循环游离DNA中的免疫球蛋白重链和T细胞受体基因重排作为急性髓系白血病患者微小残留病的检测方法

Monitoring immunoglobulin heavy chain and T-cell receptor gene rearrangement in cfDNA as minimal residual disease detection for patients with acute myeloid leukemia.

作者信息

Zhong Ling, Chen Jiao, Huang Xiaobing, Li Yanxing, Jiang Tao

机构信息

Department of Clinical Laboratory, Sichuan Academy of Medical Science, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.

Department of Hematology, Sichuan Academy of Medical Science, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.

出版信息

Oncol Lett. 2018 Aug;16(2):2279-2288. doi: 10.3892/ol.2018.8966. Epub 2018 Jun 13.

DOI:10.3892/ol.2018.8966
PMID:30008930
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6036514/
Abstract

The present study aimed to examine whether monoclonal immunoglobulin heavy chain (IGH) or T-cell receptor (TCR) gene rearrangement in cell-free DNA (cfDNA) may be used for minimal residual disease (MRD) monitoring in patients with acute myeloid leukemia (AML). Monoclonal IGH and TCR rearrangement in cfDNA were monitored in patients with AML. A total of 94 (40%) patients had monoclonal IGH or TCR rearrangements in cfDNA at diagnosis; 84% of these patients (79 cases) achieved complete remission following 1-3 courses of induction chemotherapy. Among these cases, 89.9% were negative for monoclonal IGH or TCR rearrangement in cfDNA following consolidation chemotherapies. A total of 8 patients with consistently positive monoclonal IGH or TCR rearrangement in cfDNA relapsed within 6-10 months. During follow up, 39 patients demonstrated positive monoclonal IGH or TCR rearrangement in cfDNA and relapsed. Recurrence of monoclonal IGH or TCR rearrangement in cfDNA was observed 1-3 months earlier than bone marrow relapse and 11 patients with solitary extramedullary relapse demonstrated positive monoclonal IGH or TCR rearrangement recurrence in cfDNA. In conclusion, the detection of monoclonal IGH and TCR rearrangement in cfDNA may represent a useful tool for MRD monitoring in patients with AML.

摘要

本研究旨在探讨游离DNA（cfDNA）中的单克隆免疫球蛋白重链（IGH）或T细胞受体（TCR）基因重排是否可用于急性髓系白血病（AML）患者的微小残留病（MRD）监测。对AML患者的cfDNA中的单克隆IGH和TCR重排进行了监测。共有94例（40%）患者在诊断时cfDNA中存在单克隆IGH或TCR重排；这些患者中的84%（79例）在接受1-3个疗程的诱导化疗后达到完全缓解。在这些病例中，89.9%的患者在巩固化疗后cfDNA中的单克隆IGH或TCR重排呈阴性。共有8例cfDNA中持续存在单克隆IGH或TCR重排阳性的患者在6-10个月内复发。在随访期间，39例患者cfDNA中出现单克隆IGH或TCR重排阳性并复发。cfDNA中观察到单克隆IGH或TCR重排复发比骨髓复发早1-3个月，11例孤立性髓外复发患者cfDNA中出现单克隆IGH或TCR重排复发阳性。总之，检测cfDNA中的单克隆IGH和TCR重排可能是AML患者MRD监测的一种有用工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d27a/6036514/3d6fbcec289d/ol-16-02-2279-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d27a/6036514/bb4f59a02f92/ol-16-02-2279-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d27a/6036514/04c0bfd8ad1e/ol-16-02-2279-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d27a/6036514/8d468488e1b7/ol-16-02-2279-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d27a/6036514/3d6fbcec289d/ol-16-02-2279-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d27a/6036514/bb4f59a02f92/ol-16-02-2279-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d27a/6036514/04c0bfd8ad1e/ol-16-02-2279-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d27a/6036514/8d468488e1b7/ol-16-02-2279-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d27a/6036514/3d6fbcec289d/ol-16-02-2279-g03.jpg

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2
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3
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Front Oncol. 2023 May 5;13:1164017. doi: 10.3389/fonc.2023.1164017. eCollection 2023.
4
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5
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Front Oncol. 2022 Dec 14;12:958673. doi: 10.3389/fonc.2022.958673. eCollection 2022.
6
Patient-Specific Assays Based on Whole-Genome Sequencing Data to Measure Residual Disease in Children With Acute Lymphoblastic Leukemia: A Proof of Concept Study.基于全基因组测序数据的个性化检测方法用于测量急性淋巴细胞白血病患儿的残留疾病：一项概念验证研究。
Front Oncol. 2022 Jul 5;12:899325. doi: 10.3389/fonc.2022.899325. eCollection 2022.
7
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Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2014 Apr;31(2):206-9. doi: 10.3760/cma.j.issn.1003-9406.2014.02.017.
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