Cai Jinquan
Department of Urology, Fuzhou General Hospital of Nanjing Military Command of Chinese PLA, Fuzhou, Fujian 350025, P.R. China.
Exp Ther Med. 2013 Dec;6(6):1489-1493. doi: 10.3892/etm.2013.1345. Epub 2013 Oct 14.
The aim of this study was to investigate the roles of the transcription factor Snail and adhesion factor epithelial-cadherin (E-cadherin) in clear cell renal cell carcinoma (CCRCC) and evaluate their correlation with tumor pathological grading, clinical stage, invasion and metastases. The expression of Snail and E-cadherin protein in 69 samples of CCRCC tissue, 58 samples of para-cancerous mucosa and 10 samples of normal renal tissue were detected using the immunohistochemical streptavidin-peroxidase method. The positivity rate of Snail in CCRCC was 82.61%, which was significantly higher than that in para-cancerous mucosa (43.10%, P<0.001). The positivity rate of E-cadherin in CCRCC was 31.88%, which was significantly lower than that in para-cancerous mucosa (91.38%, P<0.001). The expression of E-cadherin and Snail correlated significantly with tumor differential degree, clinical stage and the depth of tumor invasion and distant metastasis (P<0.05). There was a negative correlation between the expression of E-cadherin and Snail in CCRCC. The overexpression of Snail and reduced expression of E-cadherin may be important biological markers for the invasion and metastasis of CCRCC. The combined detection of E-cadherin and Snail has far-reaching significance for the prediction of CCRCC invasion and metastasis.
本研究旨在探讨转录因子Snail和黏附因子上皮钙黏蛋白（E-cadherin）在透明细胞肾细胞癌（CCRCC）中的作用，并评估它们与肿瘤病理分级、临床分期、侵袭和转移的相关性。采用免疫组织化学链霉亲和素-过氧化物酶法检测69例CCRCC组织样本、58例癌旁黏膜样本和10例正常肾组织样本中Snail和E-cadherin蛋白的表达。CCRCC中Snail的阳性率为82.61%，显著高于癌旁黏膜（43.10%，P<0.001）。CCRCC中E-cadherin的阳性率为31.88%，显著低于癌旁黏膜（91.38%，P<0.001）。E-cadherin和Snail的表达与肿瘤分化程度、临床分期以及肿瘤侵袭深度和远处转移显著相关（P<0.05）。CCRCC中E-cadherin和Snail的表达呈负相关。Snail的过表达和E-cadherin的表达降低可能是CCRCC侵袭和转移的重要生物学标志物。E-cadherin和Snail的联合检测对预测CCRCC的侵袭和转移具有深远意义。
