Wyczółkowska J, Prouvost-Danon A, Maśliński C
Agents Actions. 1986 Apr;18(1-2):172-5. doi: 10.1007/BF01988013.
The conditions for active sensitization of hamster peritoneal and pleural mast cells and IgE-induced histamine release as well as cell desensitization were defined. Immunization of hamsters with ovalbumin (5 micrograms) in Al/OH/3 gel (5 mg) with several boosters resulted in sensitization of peritoneal and pleural mast cells; in the presence of extracellular Ca++, pH of medium 7.2 and at 37 degrees C these cells released up to 70% of histamine on the challenge with specific antigen. Partial release was observed when the cells were challenged with antigen in the absence of extracellular calcium. The rate of release is high during the first seconds of activation and is complete at 1 min. 30 min preincubation of peritoneal and pleural mast cells in calcium-free conditions (in the presence of 4 mM EDTA) resulted in complete desensitization of cells to subsequent action of antigen in optimal conditions. The present experiments demonstrate, that hamster peritoneal and pleural mast cells can be a useful model system for in vitro studies of the mechanisms of IgE-induced cell activation.
确定了仓鼠腹膜和胸膜肥大细胞主动致敏、IgE诱导组胺释放以及细胞脱敏的条件。用卵清蛋白（5微克）与Al/OH/3凝胶（5毫克）对仓鼠进行免疫，并多次加强免疫，可导致腹膜和胸膜肥大细胞致敏；在细胞外钙离子存在、培养基pH值为7.2且温度为37℃的条件下，这些细胞在受到特异性抗原攻击时可释放高达70%的组胺。当细胞在无细胞外钙的情况下受到抗原攻击时，观察到部分释放。释放速率在激活的最初几秒内较高，并在1分钟时完成。腹膜和胸膜肥大细胞在无钙条件下（存在4 mM EDTA）预孵育30分钟，可导致细胞在最佳条件下对随后的抗原作用完全脱敏。本实验表明，仓鼠腹膜和胸膜肥大细胞可作为体外研究IgE诱导细胞激活机制的有用模型系统。
