Wyczolkowska J, Rydzynski K, Prouvost-Danon A
Department of Biogenic Amines, Polish Academy of Sciences, Lodz.
Int Arch Allergy Immunol. 1992;97(2):167-72. doi: 10.1159/000236113.
Peritoneal mast cells of Syrian hamsters release histamine to the action of concanavalin A (Con A) in dose-dependent fashion. The rate of release was very rapid in the first seconds of cell activation and completed in 60 s after the challenge. Morphological changes concomitant to the lectin treatment, followed by electron microscopy, show that early signs of exocytosis are seen after 10 s. The process starts in peripherally located granules which swell, have a decreased density and form pores by fusion of the cellular membrane and the perigranular membranes. Then it spreads toward the cell interior by fusion of granules and forming intracytoplasmic cavities. Some extruded granules are also observed. Preincubation of lectin with rat IgE or with rat serum induced an inhibition of its histamine releasing action. Immunization increased the Con A-induced histamine release in young but not in older hamsters. An IgE-mediated mechanism is suggested for the parallel ultrastructural changes and histamine release effects induced by Con A on the hamster mast cell.
叙利亚仓鼠的腹膜肥大细胞以剂量依赖的方式向伴刀豆球蛋白A（Con A）的作用释放组胺。在细胞激活的最初几秒内释放速率非常快，并在刺激后60秒内完成。通过电子显微镜观察，伴随凝集素处理的形态学变化表明，在10秒后可见胞吐作用的早期迹象。该过程始于位于周边的颗粒，这些颗粒肿胀，密度降低，并通过细胞膜和颗粒周围膜的融合形成孔。然后通过颗粒融合并形成胞质内空腔向细胞内部扩散。还观察到一些挤出的颗粒。凝集素与大鼠IgE或大鼠血清预孵育会抑制其组胺释放作用。免疫增加了年轻仓鼠而非老年仓鼠中Con A诱导的组胺释放。有人提出一种IgE介导机制来解释Con A对仓鼠肥大细胞诱导的平行超微结构变化和组胺释放效应。
