Wang Yusheng, Yin Wanzhong, Zhu Xuewei
Department of Otolaryngology Head & Neck Surgery, First Hospital of Jilin University , Changchun , China.
Acta Otolaryngol. 2014 Jan;134(1):105-10. doi: 10.3109/00016489.2013.844365. Epub 2013 Nov 21.
Autophagy was involved in the radiation treatment of CNE-2 cells, and blocked autophagy enhances the radiosensitivity of nasopharyngeal carcinoma cell line CNE-2 in vitro.
To determine whether autophagy induced by radiation therapy contributes to tumor cell death or represents a mechanism of resistance to therapy-mediated cell death.
Autophagy in the CNE-2 nasopharyngeal carcinoma cells after radiation treatment was determined by quantitative GFP-LC3 analysis, electron microscopy, and autophagy-related molecules analysis by Western blotting. The contribution of autophagy to the cell viability was determined by MTT assay and clonogenic assay.
Autophagy was involved in CNE-2 cells post radiation treatment, and autophagy could ameliorate the cell viability post radiation. On the other hand, inhibition of autophagy could reduce cell viability and decrease the cell survival.
自噬参与了CNE-2细胞的放射治疗，阻断自噬可增强鼻咽癌细胞系CNE-2在体外的放射敏感性。
确定放射治疗诱导的自噬是有助于肿瘤细胞死亡还是代表对治疗介导的细胞死亡的一种抵抗机制。
通过定量GFP-LC3分析、电子显微镜以及蛋白质免疫印迹法分析自噬相关分子，来确定放射治疗后CNE-2鼻咽癌细胞中的自噬情况。通过MTT法和克隆形成试验确定自噬对细胞活力的影响。
自噬参与了放射治疗后的CNE-2细胞，且自噬可改善放射后的细胞活力。另一方面，抑制自噬可降低细胞活力并减少细胞存活。
