Garfield R E
Am J Physiol. 1986 Jul;251(1 Pt 1):C41-54. doi: 10.1152/ajpcell.1986.251.1.C41.
Whole-mount preparations of the uterus and mesentery from nonpregnant rats were examined after staining with glyoxylic acid or acetylcholinesterase to demonstrate the innervation. Some uterine tissues were also evaluated by electron microscopy. Glyoxylic acid fluorescent nerves were present in the mesentery, mesometrium, and uterine wall exclusively around blood vessels. Acetylcholinesterase positive nerve fibers from Frankenhäuser's plexus (cervical ganglia) were associated with blood vessels and muscle in the uterine wall but not in the mesentery. Electron microscopy revealed nerve varicosities with granular vesicles associated with blood vessels and varicosities with agranular vesicles located near blood vessels and muscle cells. Treatment of animals for 3 days with 5- and 6-hydroxydopamine, respectively, increased granular size and damaged the varicosities with granular vesicles but did not change nerves with agranular vesicles or induce the presence of gap junctions. The results of this study suggest that blood vessels in the uterus are highly innervated by both adrenergic and other types of nerves that probably control blood flow. Nonadrenergic but not adrenergic nerves may also directly control myometrial contractility.
用乙醛酸或乙酰胆碱酯酶染色后，对未怀孕大鼠的子宫和肠系膜进行整装制片，以显示神经支配情况。部分子宫组织也通过电子显微镜进行评估。乙醛酸荧光神经仅存在于肠系膜、子宫系膜和子宫壁中围绕血管的区域。来自Frankenhäuser神经丛（宫颈神经节）的乙酰胆碱酯酶阳性神经纤维与子宫壁中的血管和肌肉相关，但在肠系膜中则无。电子显微镜显示，有颗粒小泡的神经膨体与血管相关，而无颗粒小泡的膨体位于血管和肌肉细胞附近。分别用5-羟多巴胺和6-羟多巴胺对动物进行3天治疗，增加了颗粒大小并损伤了有颗粒小泡的膨体，但未改变无颗粒小泡的神经，也未诱导缝隙连接的出现。本研究结果表明，子宫中的血管受肾上腺素能神经和其他可能控制血流的神经高度支配。非肾上腺素能而非肾上腺素能神经也可能直接控制子宫肌层的收缩。
