Miciński Bartosz, Jana Barbara, Całka Jarosław
Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 14, 11-041 Olsztyn, Poland.
Division of Reproductive Biology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
Animals (Basel). 2022 Jun 29;12(13):1676. doi: 10.3390/ani12131676.
The focus of this study was based on examining the impact of endometritis on the chemical coding of the paracervical ganglion (PCG) perikaryal populations supplying pig uterus. Four weeks after the injection of Fast Blue retrograde tracer into uterine horns, either the (. ) suspension or saline solution was applied to both horns. Laparotomy treatment was performed for the control group. Uterine cervices containing PCG were extracted on the eighth day after previous treatments. Subsequent macroscopic and histopathologic examinations acknowledged the severe form of acute endometritis in the . -treated gilts, whereas double-labeling immunofluorescence procedures allowed changes to be analyzed in the PCG perikaryal populations coded with vesicular acetylcholine transporter (VAChT) and/or somatostatin (SOM), vasoactive intestinal polypeptide (VIP), a neuronal isoform of nitric oxide synthase (nNOS), galanin (GAL). The acetylcholinesterase (AChE) detection method was used to check for the presence and changes in the expression of this enzyme and further confirm the presence of cholinergic perikarya in PCG. Treatment with . resulted in an increase in VAChT+/VIP+, VAChT+/VIP-, VAChT+/SOM+, VAChT+/SOM-, VAChT+/GAL- and VAChT+/nNOS- PCG uterine perikarya. An additional increase was noted in the non-cholinergic VIP-, SOM- and nNOS-immunopositive populations, as well as a decrease in the number of cholinergic nNOS-positive perikarya. Moreover, the population of cholinergic GAL-expressing perikarya that appeared in the E. coli-injected gilts and . injections lowered the number of AChE-positive perikarya. The neurochemical characteristics of the cholinergic uterine perikarya of the PCG were altered and influenced by the pathological state (inflammation of the uterus). These results may indicate the additional influence of such a state on the functioning of this organ.
本研究的重点是考察子宫内膜炎对供应猪子宫的子宫颈旁神经节（PCG）神经元胞体群化学编码的影响。在向子宫角注射快蓝逆行示踪剂四周后，向两个子宫角分别施加（. ）悬液或盐溶液。对照组进行剖腹手术治疗。在先前治疗后的第八天，取出含有PCG的子宫颈。随后的宏观和组织病理学检查确认了在. 处理的后备母猪中存在严重形式的急性子宫内膜炎，而双重标记免疫荧光程序允许分析PCG神经元胞体群中用囊泡乙酰胆碱转运体（VAChT）和/或生长抑素（SOM）、血管活性肠肽（VIP）、神经元型一氧化氮合酶（nNOS）、甘丙肽（GAL）编码的变化。采用乙酰胆碱酯酶（AChE）检测方法检查该酶的表达情况及变化，并进一步确认PCG中胆碱能神经元胞体的存在。用. 处理导致VAChT+/VIP+、VAChT+/VIP-、VAChT+/SOM+、VAChT+/SOM-、VAChT+/GAL-和VAChT+/nNOS-PCG子宫神经元胞体增加。在非胆碱能VIP-、SOM-和nNOS免疫阳性群体中也观察到额外增加，同时胆碱能nNOS阳性神经元胞体数量减少。此外，在注射大肠杆菌的后备母猪和. 注射中出现的表达胆碱能GAL的神经元胞体群体减少了AChE阳性神经元胞体的数量。PCG胆碱能子宫神经元胞体的神经化学特征因病理状态（子宫炎症）而改变并受到影响。这些结果可能表明这种状态对该器官功能的额外影响。
