Krisman C R, Blumenfeld M L
Anal Biochem. 1986 May 1;154(2):409-13. doi: 10.1016/0003-2697(86)90006-0.
A method for the detection of glycogen synthase activity after nondenaturing polyacrylamide gel electrophoresis is described. After the electrophoretical run, the gels were incubated in situ with UDP-glucose and glycogen. Labeled or unlabeled UDP-glucose could be used, since similar activity patterns were obtained by autoradiography or iodine staining of the gels. The method here described offers several advantages in terms of speed, sensitivity, and economy when compared with other procedures.
描述了一种在非变性聚丙烯酰胺凝胶电泳后检测糖原合酶活性的方法。电泳结束后，将凝胶原位与UDP-葡萄糖和糖原一起孵育。可以使用标记或未标记的UDP-葡萄糖，因为通过凝胶的放射自显影或碘染色可获得相似的活性模式。与其他方法相比，本文所述方法在速度、灵敏度和经济性方面具有几个优点。
