Krivokobyl'skaia Ia I, Solov'eva G A
Biokhimiia. 1984 Nov;49(11):1819-27.
A glycogen-free glycogen synthase I was isolated from rabbit skeletal muscles as a tetramer. Using the light scattering technique, the formation of the glycogen synthase I--glycogen complex was investigated; the Kd value [(0.40 +/- 0.09) X 10(-7) M], the absorption capacity of glycogen towards the enzyme [aM = (1.89 +/- 0.4) X 10(-6) mol] and the number of enzyme-binding sites per polysaccharide molecule (n = 10) were determined, using rabbit liver glycogen (Mr = 5.28 X 10(6)). After the formation of the glycogen synthase I--glycogen complex has been completed, the reactivity of some SH-groups of the enzyme is reduced and some of them become masked towards 5,5'-dithiobis-2-nitrobenzoate.
从兔骨骼肌中分离出一种无糖原的糖原合酶I，它是一种四聚体。利用光散射技术研究了糖原合酶I - 糖原复合物的形成；使用兔肝糖原（Mr = 5.28×10⁶）测定了Kd值[（0.40±0.09）×10⁻⁷ M]、糖原对该酶的吸附容量[aM =（1.89±0.4）×10⁻⁶ mol]以及每个多糖分子的酶结合位点数（n = 10）。在糖原合酶I - 糖原复合物形成完成后，该酶的一些SH基团的反应性降低，其中一些对5,5'-二硫代双-2-硝基苯甲酸变得隐蔽。
