通过连续流动荧光法进行核酸定量

Nucleic acid quantitation by continuous flow fluorometry.

作者信息

Murray M G, Paaren H E

出版信息

Anal Biochem. 1986 May 1;154(2):638-42. doi: 10.1016/0003-2697(86)90040-0.

Abstract

A system for rapid and sensitive fluorometric measurement of nucleic acids is described. Samples can be analyzed virtually as fast as they can be injected into the analyzer (three to five per minute). 5-microliter samples ranging in concentration from 0.05 to 40 micrograms/ml can be measured accurately. The sample can easily be recovered.

摘要

描述了一种用于核酸快速灵敏荧光测量的系统。样品几乎可以在注入分析仪后立即进行分析（每分钟三到五个）。可以准确测量浓度范围为0.05至40微克/毫升的5微升样品。样品可以轻松回收。