通过流动注射分析对粗组织提取物中的DNA和RNA进行定量分析。

Quantitation of DNA and RNA in crude tissue extracts by flow injection analysis.

作者信息

Caldarone E M, Buckley L J

机构信息

National Oceanic and Atmospheric Administration, Narragansett Laboratory, Rhode Island 02882-1199.

出版信息

Anal Biochem. 1991 Nov 15;199(1):137-41. doi: 10.1016/0003-2697(91)90281-w.

DOI:10.1016/0003-2697(91)90281-w

PMID:1725474

Abstract

An automated two-dye flow injection analysis system to quantitate DNA and RNA in crude extracts of tissues is described. The method uses the fluorochrome dyes ethidium bromide and Hoechst 33258. DNA concentration is determined directly from its fluorescence in Hoechst dye. RNA is estimated from fluorescence in ethidium bromide after subtraction of the fluorescence due to DNA. This method has several advantages: a simple extraction procedure, a low detection limit (0.01 micrograms DNA and 0.10 micrograms RNA), automation, and a high sample throughput.

摘要

本文描述了一种用于定量组织粗提物中DNA和RNA的自动化双染料流动注射分析系统。该方法使用荧光染料溴化乙锭和Hoechst 33258。DNA浓度直接由其在Hoechst染料中的荧光测定。RNA通过扣除DNA产生的荧光后，由溴化乙锭中的荧光估算得出。该方法具有几个优点：提取过程简单、检测限低（0.01微克DNA和0.10微克RNA）、自动化以及样品通量高。