National Institute of Genetics, 411, Misima, Japan.
Theor Appl Genet. 1984 Aug;68(5):467-73. doi: 10.1007/BF00254822.
In order to examine the effects of different alleles on the gene expression at the waxy locus, the Wx gene product which controls the synthesis of amylose was isolated from endosperm starch of rice plants and analysed by electrophoretic techniques. The major protein bound to starch granules was absent in most of waxy strains and increased with the number of Wx alleles in triploid endosperms, suggesting that the major protein is the Wx gene product. In addition to wx alleles which result in the absence or drastic reduction of the Wx gene product and amylose, differentiation of Wx alleles seemed to have occurred among nonwaxy rice strains. At least two Wx alleles with different efficiencies in the production of the major protein as well as amylose were detected. These alleles are discussed in relation to regulation of the gene expression.
为了研究不同等位基因对蜡质基因表达的影响，我们从水稻胚乳淀粉中分离出控制直链淀粉合成的 Wx 基因产物，并通过电泳技术进行分析。大多数蜡质品种的淀粉粒结合的主要蛋白质缺失，而在三倍体胚乳中，随着 Wx 等位基因数量的增加，该蛋白质增加，这表明该主要蛋白质是 Wx 基因产物。除了 wx 等位基因导致 Wx 基因产物和直链淀粉缺失或大幅减少之外，非蜡质水稻品种的 Wx 等位基因似乎也发生了分化。至少检测到两个在产生主要蛋白质和直链淀粉方面效率不同的 Wx 等位基因。这些等位基因与基因表达的调控有关。
