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日本北海道非糯稻品种中负责淀粉特性的遗传区域之间的相互作用。

Interaction between genetic regions responsible for the starch properties in non-glutinous rice varieties in Hokkaido, Japan.

作者信息

Ikegaya Tomohito

机构信息

Institute of Crop Science, National Agricultural and Food Research Organization, 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan.

出版信息

Breed Sci. 2024 Apr;74(2):159-165. doi: 10.1270/jsbbs.23087. Epub 2024 Mar 22.

Abstract

Starch properties are the major determinants of grain quality and food characteristics in rice ( L.). Understanding the interactions between genetic regions responsible for starch properties will lead to the development of rice cultivars with desirable characteristics. This study investigated the genetic effect and interaction between , a low-amylose quantitative trait locus (QTL), and the genetic region around (). Both these factors are responsible for the starch properties of the Hokkaido breeding population. The amylose content, pasting temperature, and amylopectin chain-length distribution were compared using F lines derived from the cross between the lower amylose content and lower pasting temperature strain 'Hokkai332 (, )' and the higher amylose content and higher pasting temperature variety 'Kitagenki (-, )'. The genotype exhibited low amylose content and reduced the hardness of boiled rice but increased the ratio of amylopectin long chains and did not alter the pasting temperature. In contrast, the genotype was associated with pasting temperature but did not affect the amylose content and hardness of boiled rice. It was suggested that appropriately selecting genotypes of these genetic regions and QTL would allow the fine-tuning of starch properties of cooked rice suitable for future demand.

摘要

淀粉特性是水稻(Oryza sativa L.)籽粒品质和食品特性的主要决定因素。了解负责淀粉特性的遗传区域之间的相互作用将有助于培育具有理想特性的水稻品种。本研究调查了低直链淀粉含量数量性状位点(QTL)ALK以及ALK周围遗传区域的遗传效应和相互作用。这两个因素都对北海道育种群体的淀粉特性有影响。利用低直链淀粉含量和低糊化温度品系‘北海332(ALK,ALK)’与高直链淀粉含量和高糊化温度品种‘北辉锦(ALK,ALK)’杂交得到的F2代株系,比较了直链淀粉含量、糊化温度和支链淀粉链长分布。ALK基因型表现出低直链淀粉含量,降低了米饭的硬度,但增加了支链淀粉长链的比例,且未改变糊化温度。相比之下,ALK基因型与糊化温度有关,但不影响直链淀粉含量和米饭硬度。研究表明,适当选择这些遗传区域和QTL的基因型将能够微调适合未来需求的蒸煮米饭的淀粉特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dffa/11442103/10a8fd89dec1/74_159-g001.jpg

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