Biological Laboratory, Department of Plant Physiology, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV, Amsterdam, The Netherlands.
Plant Cell Rep. 1983 Feb;2(1):40-2. doi: 10.1007/BF00269233.
During callus formation a huge increase in alcoholdehydrogenase activity was observed in potato tuber tissue discs. Callus formation was no prerequisite for this increase; slicing and subsequent incubation of potato tuber tissue discs always led to an increase in alcohol dehydrogenase activity, which was dependent on cytoplasmic protein synthesis. A three-fold increase was observed during incubation in moist air (periderm formation) and during incubation on nutrient agar without carbon source. A six- to eight-fold increase occurred during incubation on nutrient agar with sucrose, ribose or pyruvate as carbon source. The extra increase in alcohol dehydrogenase activity did not occur in the presence of equimolar amounts of mannitol, sorbitol, succinate or ethanol. The extent of the activity increase was not directly correlated with the presence of a carbon source suitable for maintaining growth.
在愈伤组织形成过程中，观察到马铃薯块茎组织切片中醇脱氢酶活性大量增加。这种增加不需要愈伤组织形成；将马铃薯块茎组织切片切割并随后培养总是导致醇脱氢酶活性增加，这依赖于细胞质蛋白质合成。在潮湿空气中培养（周皮形成）和在不含碳源的营养琼脂上培养时，观察到增加了三倍。在含有蔗糖、核糖或丙酮酸作为碳源的营养琼脂上培养时，增加了六到八倍。在含有等摩尔甘露醇、山梨醇、琥珀酸或乙醇的情况下，醇脱氢酶活性不会额外增加。活性增加的程度与适合维持生长的碳源的存在没有直接关系。
