马唐豆愈伤组织的体外分化

In vitro differentiation in callus cultures of moth bean, Vigna aconitifolia (JACQ) marechal.

机构信息

Department of Botany, University of Rajasthan, 302004, Jaipur, India.

出版信息

Plant Cell Rep. 1983 Feb;2(1):47-50. doi: 10.1007/BF00269235.

Abstract

Callus cultures of two cultivars of Vigna aconitifolia (IPCMO-926, RDM-120) were raised and their growth and differentiation studied. In IPCMO-926 callus cultures, numerous shoot buds differentiated on MS medium with BA (0.4-22.2 μM) alone or in combination with IAA (5.7 μM). In RDM-120 best differentiation of shoot buds was observed on a medium with K (23.2 μM) and IAA (5.7 μM). Kinetin alone, however, induced rhizogenesis in callus cultures. In suspension cultures of IPCMO-926 embryoids differentiated on MS medium with K (0.5 μM) and 2,4-D (0.4 and 0.9 μM).

摘要

培养了两个豇豆品种（IPCMO-926、RDM-120）的愈伤组织，并研究了它们的生长和分化。在 IPCMO-926 愈伤组织培养中，单独或与 IAA（5.7 μM）一起，在含有 BA（0.4-22.2 μM）的 MS 培养基上分化出许多芽。在 RDM-120 中，在含有 K（23.2 μM）和 IAA（5.7 μM）的培养基上观察到最佳芽分化。然而，细胞分裂素单独诱导愈伤组织生根。在 IPCMO-926 的悬浮培养物中，胚状体在含有 K（0.5 μM）和 2,4-D（0.4 和 0.9 μM）的 MS 培养基上分化。