Tissue Culture for Crops Project, Department of Biology, Botany section, Colorado State University, 80523, Fort Collins, CO, USA.
Plant Cell Rep. 1988 Mar;7(2):138-41. doi: 10.1007/BF00270124.
Plant regeneration has been achieved routinely from established cell suspension culture lines of Vigna aconitifolia (moth bean), a highly drought tolerant grain legume. The cultures originated from three-week-old leaf callus. Several media including MS, B5, AA, SL, PCM, SH and L-6 were tested for their effects on cell growth. Maximum growth was observed in L-6 medium containing 44.5 μM 2,4-D. After 6 to 8 weeks the suspensions were filtered through 500, 250, 125 and 60 μm sieves, respectively, for four to five subcultures. An embryogenic cell line (VA-686) was obtained from the cell fraction collected below 250 μm. The VA-686 cell line is being maintained on L-6 medium with 4.5 μM 2,4-D and 2.3 μM Zeatin. Somatic embryogenesis was induced by transferring the cells to L-6 medium with 4.6 μM zeatin in which green cell clusters were produced. The somatic embryos developed from most of the cell clusters when plated on L-6 agar medium with 2.3 μM BA.Plantlets were obtained from the embryos on L-6 medium with 10.0 μM IBA. The regenerated plants were grown to maturity in the greenhouse.
从高度耐旱的粮食豆类植物豇豆（moth bean）的已建立细胞悬浮培养系中已经常规实现了植物再生。这些培养物源自三周大的叶愈伤组织。对几种培养基，包括 MS、B5、AA、SL、PCM、SH 和 L-6，进行了测试，以观察它们对细胞生长的影响。在含有 44.5 μM 2,4-D 的 L-6 培养基中观察到最大生长。6 至 8 周后，将悬浮液分别通过 500、250、125 和 60 μm 的筛子过滤，进行四到五次继代培养。从 250 μm 以下收集的细胞部分获得了胚性细胞系（VA-686）。VA-686 细胞系在含有 4.5 μM 2,4-D 和 2.3 μM Zeatin 的 L-6 培养基中维持。通过将细胞转移到含有 4.6 μM Zeatin 的 L-6 培养基中，可以诱导体细胞胚胎发生，在该培养基中会产生绿色细胞团。当将细胞接种在含有 2.3 μM BA 的 L-6 琼脂培养基上时，大多数细胞团都会发育成体细胞胚胎。将胚状体接种在含有 10.0 μM IBA 的 L-6 培养基上，从胚状体中获得了植株。再生植物在温室中生长成熟。
