抗旱豆类作物的研究：兵豆，鹰咀豆(Vigna aconitifolia (Jacq) marechal)。I. 原生质体培养和器官发生。

Studies on a drought resistant legume: The moth bean, Vigna aconitifolia (Jacq) marechal. I. Protoplast culture and organogenesis.

机构信息

Biochemistry Division, National Chemical Laboratory, 411 008, Poona, India.

出版信息

Plant Cell Rep. 1984 Feb;3(1):30-2. doi: 10.1007/BF00270225.

Abstract

High yields of viable protoplasts were obtained from callus cultures derived from shoot apices of Vigna aconitifolia (JACQ) Marechal. The protoplasts divided and formed cell clusters on modified MS medium. The protoplast-derived callus formed multiple shoot buds on MS and B5 basal media without supplements, on MS medium containing supplements and on B5 medium containing charcoal (0.25%). Shoot formation occurred.

摘要

从豇豆（Vigna aconitifolia（JACQ）Marechal）茎尖愈伤组织中获得了大量有活力的原生质体。这些原生质体在改良的 MS 培养基上分裂并形成细胞团。这些原生质体衍生的愈伤组织在不含添加物的 MS 和 B5 基本培养基、含添加物的 MS 培养基以及含 0.25%活性炭的 B5 培养基上形成多个芽。