Section on Germplasm Development and Biotechnology, Agriculture Canada, Research Station, 195 Dafoe Road, R3T 2M9, Winnipeg, Manitoba, Canada.
J Chem Ecol. 1991 Aug;17(8):1543-56. doi: 10.1007/BF00984687.
Sinalbin was identified as a chemical component of insect anti-xenosis and antibiosis resistance mechanisms in seedlings ofSinapis alba by DEAE-Sephadex chromatography, HPLC, treatment with sulfatase and myrosinase, various feeding tests using artificial and natural substrates, and by measuring sinalbin concentrations in cotyledons and leaves during seedling development. The effects of sinaibin on feeding were dependent upon the insect species and upon the rapidly changing profile of sinaibin concentrations in the developing seedling. The high concentrations of sinalbin found in young cotyledons (up to 20 mM) and leaves (up to 10 mM) deterred the feeding of the flea beetle,Phyllotreta cruciferae Goeze and larvae of the bertha armyworm,Mamestra configurata Walker. The protection that sinalbin confers upon the vulnerable, newly emerged seedling (and upon tiny, young leaves) appears critical for the first few days of survival ofS. alba under feeding pressure from flea beetles in the field. The lower concentrations of sinaibin found in older cotyledons and leaves (2-3 mM) offer little or no protection againstP. cruciferae and may actually stimulate the feeding of this crucifer specialist. These concentrations of sinaibin, however, are still effective in reducing the level of feeding by larvae of the more generalist feederM. configurata.
通过 DEAE-Sephadex 层析、HPLC、用硫酸酯酶和糜蛋白酶处理、使用人工和天然基质进行各种饲养试验，以及测量在幼苗发育过程中种皮苷在子叶和叶片中的浓度，确定了白芥幼苗中一种化学成分为昆虫抗异生物和抗生物防御机制的物质，即为白芥子甙。种皮苷对摄食的影响取决于昆虫种类和种皮苷在发育中的幼苗中浓度的快速变化情况。在幼嫩的子叶（高达 20mM）和叶片（高达 10mM）中发现的高浓度的种皮苷阻止了跳蚤甲虫 Phyllotreta cruciferae Goeze 和幼虫 bertha 粘虫 Mamestra configurata Walker 的取食。种皮苷为脆弱的、新出现的幼苗（以及小而嫩的叶片）提供的保护，似乎对于白芥在田间受到跳蚤甲虫摄食压力下的最初几天的生存至关重要。在较老的子叶和叶片中发现的浓度较低的种皮苷（2-3mM）对 Phyllotreta cruciferae 和实际上可能刺激这种十字花科专家的取食提供的保护作用很小或没有。然而，这些浓度的种皮苷仍然可以有效地降低更一般的食者 Mamestra configurata 幼虫的取食水平。
