在乙酰丙酸处理的 Cyanidium caldarium 中，血红素掺入藻蓝胆素。
Incorporation of haem into phycocyanobilin in levulinic acid treated Cyanidium caldarium.
机构信息
Botanisches Institut der Universität München, Menzinger Straße 67, D-8000, München 19, FRG.
出版信息
Plant Cell Rep. 1983 Apr;2(2):85-7. doi: 10.1007/BF00270172.
Cells of the red alga Cyanidium caldarium were preincubated with 5 mmol 1(-1) levulinic acid (LA) and subsequently incubated with (14)C-labelled haem (5.67 Bq nmol(-1)). Phycocyanin was isolated. The specific radio-activity of its chromophore phycocyanobilin (PCB) was determined after cleavage and purification by thin layer chromatography. The percentage of PCB formed from labelled haem within 0.5 h was considerably higher in LA treated cells than in non-treated controls. This difference disappeared after prolonged incubation (16.5 h) with haem. The results are interpreted as possible incorporation of haem into preexisting apoprotein.
将红色海藻 Cyanidium caldarium 的细胞用 5mmol1(-1) 的乙酰丙酸（LA）进行预孵育，然后用（14)C 标记的血红素（5.67 Bq nmol(-1))孵育。分离藻蓝蛋白。用薄层层析法裂解和纯化后，测定其发色团藻蓝胆素（PCB）的比放射性。在 LA 处理的细胞中，与未经处理的对照相比，在 0.5 小时内从标记的血红素形成的 PCB 的百分比明显更高。在用血红素孵育 16.5 小时后，这种差异消失了。这些结果可以解释为血红素可能掺入到预先存在的脱辅基蛋白中。
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