Brown S B, Holroyd A J, Troxler R F
Biochem J. 1980 Aug 15;190(2):445-9. doi: 10.1042/bj1900445.
The origin of the lactam oxygen atoms of phycocyanobilin from Cyanidium caldarium was studied using 18O labelling. By inhibiting photosynthesis, a high 18O enrichment was maintained in the gas phase and the resulting incorporation of label showed that the lactam oxygen atoms were derived from two oxygen molecules. Slow exchange of these oxygen atoms with water was demonstrated directly by using H218O.
利用18O标记研究了来自嗜热蓝藻的藻蓝胆素内酰胺氧原子的来源。通过抑制光合作用,气相中保持了较高的18O富集,标记物的掺入结果表明内酰胺氧原子来源于两个氧分子。使用H218O直接证明了这些氧原子与水的缓慢交换。