金鸡纳属组织培养及细胞悬浮培养中生物碱的生产研究。

Studies on tissue cultures of the genus Cinchona L. alkaloid production in cell suspension cultures.

机构信息

Akademie der Wissenschaften der DDR, Zentralinstitut für Genetik und Kulturpflanzenforschung, DDR-4325, Gatersleben.

出版信息

Plant Cell Rep. 1983 Jun;2(3):122-5. doi: 10.1007/BF00269334.

Abstract

Initiation and culture of callus and cell suspensions of Cinchona ledgeriana and C. succirubra as well as the successful isolation and selection of a high-yielding alkaloid-forming strain derived from the leaf rachis of a C. succirubra plant are described. Results of feeding experiments with L-tryptophan using two different culture procedures are presented and discussed. Maximum alkaloid yields of up to 0.9% (based on dry weight) or 6.35 mg/l have been obtained.

摘要

本文描述了金鸡纳 ledgeriana 和 C. succirubra 的愈伤组织和细胞悬浮液的起始和培养，以及从 C. succirubra 植物的叶轴成功分离和选择高产生物碱形成菌株的过程。本文介绍并讨论了使用两种不同培养方法进行 L-色氨酸喂养实验的结果。已获得高达 0.9%（基于干重）或 6.35mg/l 的最大生物碱产量。