从玉米未成熟胚培养的叶组织体外再生植株。

Plant regeneration in vitro from leaf tissues derived from cultured immature embryos of Zea mays L.

机构信息

International Plant Research Institute, 830 Bransten Road, 94070, San Carlos, CA, USA.

出版信息

Plant Cell Rep. 1983 Aug;2(4):183-5. doi: 10.1007/BF00270098.

Abstract

Maize (Zea mays L.) A188 calluses derived from leaf tissues of in vitro grown seedlings were initiated and maintained on Murashige and Skoog medium supplemented with 2 mg/1 2,4-dichlorophenoxyacetic acid (2,4-D). The calluses produced green leafy structures and subsequently plantlets upon transfer to N6 medium containing 2 mg/1 2,4-D and 0.1 mg/1 zeatin. An embryo-like structure with a green prominent coleoptile and a scutellum-like body was also obtained.

摘要

玉米（Zea mays L.）A188 愈伤组织由离体培养的幼苗叶片组织诱导并在添加 2 mg/1 2,4-二氯苯氧乙酸（2,4-D）的 Murashige 和 Skoog 培养基上维持。这些愈伤组织产生了绿色的叶片状结构，随后在含有 2 mg/1 2,4-D 和 0.1 mg/1 玉米素的 N6 培养基上转移时，形成了小植株。还获得了一个具有绿色明显胚根鞘和盾状器官的胚状体结构。