叶片组织诱导菽麻体细胞胚胎发生和植株再生

Somatic embryogenesis and plant regeneration from leaf tissues of Panicum maximum Jacq.

机构信息

Department of Botany, University of Florida, Gainesville, USA.

出版信息

Theor Appl Genet. 1981 Sep;59(5):275-80. doi: 10.1007/BF00264979.

Abstract

Somatic embryogenesis was induced in proliferating leaf segments of Panicum maximum Jacq., cultured on Murashige and Skoog's nutrient medium containing 2,4-dichlorophenoxyacetic acid and coconut milk. The embryoids gave rise to plants on a medium containing gibberellic acid. The plants were successfully transplanted to soil and grown to maturity. Histological examination of proliferating leaves showed that the embryogenic callus tissue was formed by divisions in cells of the lower epidermis as well as the mesophyll tissue. The regenerated plants showed the normal chromosome number of 2n=4x=32.

摘要

丛生芽叶片诱导愈伤组织产生体细胞胚胎发生在牧草地上 Jacq.，在 Murashige 和 Skoog 的含有 2,4-二氯苯氧乙酸和椰子奶的营养培养基上培养。胚状体在含有赤霉素的培养基上产生植物。植物成功地移植到土壤中并生长成熟。增殖叶片的组织学检查表明，胚性愈伤组织是由下表皮细胞以及叶肉组织的细胞分裂形成的。再生植株表现出正常的染色体数 2n=4x=32。