Department of Applied Microbiology, Institute of Microbiology, Hebrew University - Hadassah Medical School, P.O.B. 1172, 91010, Jerusalem, Israel.
Plant Cell Rep. 1983 Aug;2(4):219-22. doi: 10.1007/BF00270109.
In the present work we examined the potential benefits of the continuous culture (chemostat) technique at improving biomass yields of Mentha and Dioscorea cells and product formation (diosgenin) by Dioscorea cells. In contrast to Mentha cells, Dioscorea cells were sensitive to mechanical agitation in the exponential growth phase and could only be grown in a bubble column type fermentor. Maximal biomass yield of 0.5 and 0.4 g cell dry weight g(-1)sucrose were obtained for Mentha and Dioscorea cells, respectively. When the phosphate concentration during the growth phase of Dioscorea was increased, a maximal concentration of 7.8% diosgenin (of dry weight) was obtained. Productivity of diosgenin was 12 mg 1(-1) day(-1) in a two-stage continuous process as compared to 7.3 mg 1(-1) day(-1) in a batch culture.
在本工作中，我们考察了连续培养（恒化器）技术在提高薄荷和薯蓣细胞生物量产量和薯蓣细胞产物（薯蓣皂苷元）形成方面的潜力。与薄荷细胞不同，薯蓣细胞在指数生长期对机械搅拌敏感，只能在鼓泡塔式发酵罐中生长。薄荷和薯蓣细胞的最大生物量产量分别为 0.5 和 0.4 g 细胞干重 g(-1) 蔗糖。当薯蓣生长阶段的磷酸盐浓度增加时，可获得 7.8%（干重）的最大薯蓣皂苷元浓度。在两段式连续过程中，薯蓣皂苷元的生产率为 12 mg 1(-1) day(-1)，而在分批培养中为 7.3 mg 1(-1) day(-1)。
