植物激素的免疫分析：I. gibberellin A1、A3、A4、A7、A9 和 A20 的放射免疫分析。
The immunoassay of gibberellins : I. radioimmunoassays for the gibberellins A1, A 3, A 4, A 7, A 9 and A 20.
机构信息
Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, Postfach 102148, D-4630, Bochum, Federal Republic of Germany.
出版信息
Planta. 1983 Jan;159(1):1-6. doi: 10.1007/BF00998806.
Immunological assays for the detection of picogram amounts of gibberellins A1, A3, A4, A7, A9 and A20 are described. Antisera of high affinity (Ka over 10(10) l mol(-1)) were raised in rabbits by immunization against carboxyl-coupled gibberellin-bovine-serum-albumin conjugates and used in combination with tritiated gibberellins or gibberellin derivatives of high specific radioactivity (over 10(14) Bq mol(-1)). The assays allow gibberellin quantitation in as little as 1 mg of vegetative plant tissue.
本文描述了检测赤霉素 A1、A3、A4、A7、A9 和 A20 的微克级的免疫分析方法。通过用羧基偶联赤霉素-牛血清白蛋白缀合物免疫兔子，制备了具有高亲和力（Ka 超过 10(10) l mol(-1))的抗血清，并与氚标记赤霉素或高比活度（超过 10(14) Bq mol(-1)）的赤霉素衍生物一起使用。该测定方法允许在低至 1 毫克的植物组织中定量检测赤霉素。
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