Division of Breeding and Plant Genetics, Agricultural Research Organization, The Volcani Center, 50-250, Bet Dagan, Israel.
Plant Cell Rep. 1983 Oct;2(5):252-4. doi: 10.1007/BF00269153.
Root cultures from several species of the Solanaceae were initiated and subcultured on Murashige and Skoog medium without growth regulators. Direct shoot regeneration was observed in four different species. The effect of several concentrations of auxin (IAA) and cytokinins (BAP, zeatin) on the number of shoots generated by two highly responsive species (Nicotiana exigua, N. debneyi) is described.
从茄科的几个物种中诱导并继代培养根培养物,培养基为 Murashige 和 Skoog 培养基,不含生长调节剂。在四个不同的物种中观察到了直接芽再生。描述了几种生长素(IAA)和细胞分裂素(BAP、玉米素)浓度对两个高反应性物种(Nicotiana exigua、N. debneyi)产生的芽数量的影响。