Tanaka K, Kanno Y, Kudo S, Suzuki M
Aomori Green BioCenter, 221-10, Yamaguchi, Nogi, Aomori 030-0142, Japan e-mail:
Plant Cell Rep. 2000 Oct;19(10):946-953. doi: 10.1007/s002990000225.
Somatic embryogenesis was observed in ray-floret explants of Dendranthema grandiflorum (Ramat.) Kitamura cv. Aboukyu on Murashige and Skoog medium containing high concentrations of 3-indoleacetic acid (IAA) and kinetin. 1-Naphthaleneacetic acid also induced somatic embryogenesis but indole-3-butyric acid or 2,4-dichlorophenoxy acetic acid did not. Other cytokinins, such as 6-benzylaminopurine (BAP) and thidiazuron, were also not effective. No embryos were seen at lower IAA concentrations with kinetin and various concentrations of BAP, although higher BAP concentrations yielded many adventitious shoots. In contrast, no somatic embryogenesis was observed from leaves using any combination of plant growth regulators. Histologically, primordia showed a typical embryo shape with a well-developed vascular bundle between the shoot and the root primordia. Embryos had both stomata cells and a root system with polarity. Plants were efficiently regenerated from ray floret-derived embryos subcultured in the appropriate medium.
在含有高浓度3-吲哚乙酸（IAA）和激动素的Murashige和Skoog培养基上，在大花菊（Ramat.）北村cv.阿波久的舌状花外植体中观察到体细胞胚胎发生。1-萘乙酸也能诱导体细胞胚胎发生，但吲哚-3-丁酸或2,4-二氯苯氧乙酸则不能。其他细胞分裂素，如6-苄基氨基嘌呤（BAP）和噻苯隆，也无效。在较低的IAA浓度下，添加激动素和不同浓度的BAP时未观察到胚胎，尽管较高浓度的BAP产生了许多不定芽。相反，使用任何植物生长调节剂组合，从叶片中均未观察到体细胞胚胎发生。组织学上，原基呈现出典型的胚胎形状，在茎和根原基之间有发育良好的维管束。胚胎既有气孔细胞，又有具极性的根系。在合适的培养基中继代培养的舌状花来源的胚胎能够高效再生植株。
