Max-Planck-Institut für Biochemie, D-8033, Martinsried, Federal Republic of Germany.
Planta. 1983 Nov;159(3):216-21. doi: 10.1007/BF00397527.
Using three different techniques, isopentenyladenosine was identified and quantitated in an anise cell line (Pimpinella anisum L.), growing on a medium without cytokinin. A method to quantitate cytokinins was developed which utilizes extraction of cytokinins in the presence of a deuterated reference compound and subsequent quantitation of the cytokinins by single-ion monitoring on a gas chromatography-mass spectrometry system. The results were compared with those obtained by radioimmunoassay as well as a bioassay. Quantitations by gas chromatography-mass spectrometry and radioimmunoassay correlated well, whereas the estimates by the bioassay gave considerably lower values.
采用三种不同的技术，在不含细胞分裂素的培养基中生长的大茴香细胞系（Pimpinella anisum L.）中鉴定和定量了异戊烯基腺苷。开发了一种定量细胞分裂素的方法，该方法利用在氘标记参比化合物存在下提取细胞分裂素，然后通过气相色谱-质谱联用系统对细胞分裂素进行单离子监测进行定量。结果与放射免疫测定和生物测定的结果进行了比较。气相色谱-质谱和放射免疫测定的定量结果相关性良好，而生物测定的估计值则低得多。
