Max-Planck-Institut für Biochemie, D-8033, Martinsried, Federal Republic of Germany.
Planta. 1984 May;161(3):246-8. doi: 10.1007/BF00982920.
A cell culture of anise was grown in the presence or absence of 2,4-dichlorophenoxyacetic acid (2,4-D). Application of isopentenyladenine or isopentenyladenosine (4·10(-8) to 4·10(-7) M) to the proembryonic culture (+2,4-D) yielded an increase of the cell density, in contrast to a proembryonic culture grown without exogenous application of cytokinins. Embryogenesis was induced by transferring the cells to a hormone-free medium. Embryo development was promoted by isopentenyladenine and isopentenyladenosine (5·10(-8) to 5·10(-7) M), higher concentrations (5·10(-6) M) inhibited embryogenesis. The effect of cytokinins on embryogenesis was only promotive until the third day of culture, i.e. coincident with cell growth rather than differentiation.
茴香细胞培养在 2,4-二氯苯氧乙酸（2,4-D）的存在或不存在的情况下进行。异戊烯腺嘌呤或异戊烯腺苷（4·10（-8）至 4·10（-7）M）应用于原胚培养（+2,4-D）会导致细胞密度增加，与未外源应用细胞分裂素的原胚培养形成对比。胚胎发生通过将细胞转移到无激素培养基中诱导。异戊烯腺嘌呤和异戊烯腺苷（5·10（-8）至 5·10（-7）M）促进胚胎发育，较高浓度（5·10（-6）M）抑制胚胎发生。细胞分裂素对胚胎发生的影响仅在培养的第三天是促进的，即与细胞生长而不是分化一致。
