Mazón María José, Gómez Ana, Yilmaz Ozlem, Carrillo Manuel, Zanuy Silvia
Department of Fish Physiology and Biotechnology, Instituto de Acuicultura de Torre la Sal (IATS), Consejo Superior de Investigaciones Científicas (CSIC), Ribera de Cabanes, Castellón, Spain.
Biol Reprod. 2014 Jan 16;90(1):6. doi: 10.1095/biolreprod.113.110569. Print 2014 Jan.
Follicle-stimulating hormone (Fsh) is thought to act early in the process of spermatogenesis; however, its action in fish has not yet been clearly established. In the present work, we analyzed the effects of recombinant Fsh in sea bass (Dicentrarchus labrax) spermatogenesis according to two different approaches: direct injection of recombinant single-chain Fsh hormone (scFSH) and injection of scFSH coding sequence. Both approaches were efficient in increasing plasma Fsh at 7 and 15 days, respectively, after injection. The Fsh increment caused a significant increase in plasma 11-ketotestosterone levels and induced dramatic changes at the testicular level. Fsh-treated groups showed an increase in germ cell proliferation at Day 7, and cysts of spermatocytes and spermatids were observed at the end of the experiment. After treatment with Fsh, a suppression in amh transcripts and an increase of lhr transcripts were detected at Day 7 and Day 15, respectively, and an increment in fshr expression became evident at Day 23. These results show that Fsh initiates germ cell proliferation, triggering spermatogenesis in sea bass via androgen production and regulation of spermatogenesis-related genes.
促卵泡激素（Fsh）被认为在精子发生过程的早期起作用；然而，其在鱼类中的作用尚未明确确立。在本研究中，我们根据两种不同方法分析了重组Fsh对海鲈（欧洲鲈）精子发生的影响：直接注射重组单链Fsh激素（scFSH）和注射scFSH编码序列。两种方法分别在注射后7天和15天有效地提高了血浆Fsh水平。Fsh的增加导致血浆11-酮睾酮水平显著升高，并在睾丸水平引起显著变化。Fsh处理组在第7天生殖细胞增殖增加，实验结束时观察到精母细胞和精子细胞囊肿。用Fsh处理后，分别在第7天和第15天检测到amh转录本的抑制和lhr转录本的增加，并且在第23天fshr表达明显增加。这些结果表明，Fsh启动生殖细胞增殖，通过雄激素产生和精子发生相关基因的调控触发海鲈的精子发生。
