Gas chromatographic-mass spectroscopic identification and quantification of cyclic guanosine-3': 5'-monophosphate in maize seedlings (Zea mays).

Gas chromatographic-mass spectroscopic evidence is presented for the presence of guanosine-3': 5'-monophosphate (cGMP) in maize seedlings. The amount of cGMP (35-72 pmol g(-1) fresh weight) was quantified as a tetra-silyl derivative using gas-chromatographic detection with reference to a silylated standard of authentic cGMP. Gas-chromatographic separation of tri-silyl adenosine-3': 5'-monophosphate and tetra-silyl cGMP is demonstrated.