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用气相色谱-质谱联用法鉴定和定量分析玉米幼苗（玉米）中环鸟苷-3':5'-单磷酸。

Gas chromatographic-mass spectroscopic identification and quantification of cyclic guanosine-3': 5'-monophosphate in maize seedlings (Zea mays).

机构信息

Institut für Pharmazeutische Biologie der Universität, Schänzlestrasse 1, D-7800, Freiburg, Federal Republic of Germany.

出版信息

Planta. 1983 Nov;159(4):382-5. doi: 10.1007/BF00393178.

DOI:10.1007/BF00393178
PMID:24258237
Abstract

Gas chromatographic-mass spectroscopic evidence is presented for the presence of guanosine-3': 5'-monophosphate (cGMP) in maize seedlings. The amount of cGMP (35-72 pmol g(-1) fresh weight) was quantified as a tetra-silyl derivative using gas-chromatographic detection with reference to a silylated standard of authentic cGMP. Gas-chromatographic separation of tri-silyl adenosine-3': 5'-monophosphate and tetra-silyl cGMP is demonstrated.

摘要

本文提供了玉米幼苗中鸟苷-3':5'-一磷酸（cGMP）存在的气相色谱-质谱证据。采用气相色谱检测，参照经硅烷化处理的真实 cGMP 标准品，将 cGMP（35-72 pmol g(-1) 鲜重）定量为四硅烷衍生物。证明了三硅烷腺苷-3':5'-一磷酸和四硅烷 cGMP 的气相色谱分离。

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Gas chromatographic-mass spectroscopic identification and quantification of cyclic guanosine-3': 5'-monophosphate in maize seedlings (Zea mays).用气相色谱-质谱联用法鉴定和定量分析玉米幼苗（玉米）中环鸟苷-3':5'-单磷酸。
Planta. 1983 Nov;159(4):382-5. doi: 10.1007/BF00393178.
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本文引用的文献

1
Evidence against the occurrence of adenosine-3':5'-cyclic monophosphate in higher plants.没有证据表明腺嘌呤 3':5'-环单磷酸存在于高等植物中。
Planta. 1974 Sep;118(3):241-58. doi: 10.1007/BF00384780.
2
Cyclic nucleotides in higher plants?高等植物中的环核苷酸？
Adv Cyclic Nucleotide Res. 1974;4(0):439-60.
3
Mass spectrometry of nucleic acid components. Trimethylsilyl derivatives of nucleotides.核酸成分的质谱分析。核苷酸的三甲基硅烷基衍生物。
J Am Chem Soc. 1971 Feb 24;93(4):1014-23. doi: 10.1021/ja00733a039.
4
Cyclic GMP metabolism and involvement in biological regulation.环鸟苷酸代谢及其在生物调节中的作用。
Annu Rev Biochem. 1977;46:823-96. doi: 10.1146/annurev.bi.46.070177.004135.