Department of Physiology and Environmental Science, University of Nottingham School of Agriculture, Sutton Bonington, LE12 5RD, Loughborough, Leics., UK.
Planta. 1984 Feb;160(2):164-7. doi: 10.1007/BF00392865.
The effect of two mutations of the tomato known as Never ripe (Nr) and ripening inhibitor (rin) on abscission of the flowers was investigated. In the presence of ethylene the rate of abscission of normal and rin explants was similar, while that of Nr explants was delayed. The appearance and subsequent increases in both polygalacturonase (EC 3.2.1.15) and β-1-4-glucanase (EC 3.2.1.4) enzyme activities were similar in normal and rin explants, but retarded in Nr explants. Of these two, only polygalacturonase activity was exclusively associated with abscission-zone tissue.
研究了番茄的两个突变体(即 Never ripe(Nr)和 ripening inhibitor(rin))对花脱落的影响。在乙烯存在的情况下,正常和 rin 外植体的脱落率相似,而 Nr 外植体的脱落则延迟。正常和 rin 外植体中均出现并随后增加了多聚半乳糖醛酸酶(EC 3.2.1.15)和β-1-4-葡聚糖酶(EC 3.2.1.4)的活性,但 Nr 外植体中的活性则延迟。在这两种酶中,只有多聚半乳糖醛酸酶活性与脱落区组织特异性相关。