MSU-DOE Plant Research Laboratory, Michigan State University, 48824, East Lansing, MI, USA.
Planta. 1984 Mar;160(3):276-80. doi: 10.1007/BF00402866.
Protoplasts isolated from leaves of peas (Pisum sativum L.) and of Vicia faba L. produced 1-aminocyclopropane-1-carboxylic acid (ACC) from endogenous substrate. Synthesis of ACC and conversion of ACC to ethylene was promoted by light and inhibited by 3-(3,4-dichlorophenyl)-1,1-dimethylurea and carbonyl cyanide m-chlorophenylhydrazone. Aminoethoxyvinylglycine inhibited ethylene synthesis to a minor extent when given during incubation of the protoplasts but was very effective when added both to the medium in which the protoplasts were isolated and to the incubation medium as well. Radioactivity from [U-(14)C]methionine was incorporated into ACC and ethylene. However, the specific radioactivity of the C-2 and C-3 atoms of ACC, from which ethylene is formed, increased much faster than the specific radioactivity of ethylene. It appears that ACC and ethylene are synthesized in different compartments of the cell and that protoplasts constitute a suitable system to study this compartmentation.
从豌豆 (Pisum sativum L.) 和蚕豆 (Vicia faba L.) 的叶片中分离出的原生质体可以利用内源性底物产生 1-氨基环丙烷-1-羧酸 (ACC)。ACC 的合成和 ACC 向乙烯的转化受到光照的促进，而受到 3-(3,4-二氯苯基)-1,1-二甲基脲和羰基氰化物 m-氯苯腙的抑制。当添加到原生质体孵育过程中时，氨基乙氧基乙烯基甘氨酸对乙烯合成的抑制作用较小，但当同时添加到用于分离原生质体的培养基和孵育培养基中时，其抑制作用非常有效。[U-(14)C]蛋氨酸的放射性掺入到 ACC 和乙烯中。然而，ACC 中形成乙烯的 C-2 和 C-3 原子的比放射性增加速度比乙烯的比放射性快得多。这表明 ACC 和乙烯是在细胞的不同隔室中合成的，并且原生质体构成了研究这种隔室化的合适系统。
