Tissera Preeni, Ayres P G
Department of Biological Sciences, University of Lancaster, Lancaster LA1 4YQ, Lancashire, UK.
New Phytol. 1986 Sep;104(1):53-61. doi: 10.1111/j.1469-8137.1986.tb00633.x.
Rust infection of proximal halves of leaves of Vicia faba L. potentiates the sustained production of ethylene by abiotically wounded but uninfected distal regions. When epidermal strips were isolated from distal regions, either before or after incubation in aqueous media, stomatal opening was inhibited compared to that in strips from leaves of healthy, control plants. This inhibition, and the even greater inhibition seen in strips from rusted tissues, was reversed to differing extents by inclusion in the medium of inhibitors of the action of ethylene, namely silver ions (6 × 10 mol m ), or of its biosynthesis, namely cobalt ions and AVG (both at 1 × 10 mol m ). When ACC, a precursor of ethylene, was supplied to healthy leaves it promoted ethylene evolution after wounding and inhibited stomatal opening in strips isolated from those leaves. The cytokinins zeatin and kinetin (1 × 10 mol m ) also promoted opening in strips from both healthy and rusted regions of infected leaves, but only fusicoccin (1 × 10 mol m ) promoted wide opening in rusted regions. It is proposed that endogenous ethylene is an effective regulator of stomatal behaviour in epidermal strips prepared from either healthy or rusted regions of infected leaves of V. faba.
蚕豆叶片近端一半的锈菌感染增强了未感染的远端区域因非生物损伤而持续产生的乙烯。当在水性介质中孵育之前或之后从远端区域分离表皮条时，与来自健康对照植物叶片的条相比，气孔开放受到抑制。通过在培养基中加入乙烯作用抑制剂即银离子（6×10⁻⁶mol m⁻³）或其生物合成抑制剂即钴离子和氨基乙氧基乙烯基甘氨酸（两者均为1×10⁻⁵mol m⁻³），这种抑制以及在来自生锈组织的条中观察到的更大抑制在不同程度上得到逆转。当将乙烯前体1-氨基环丙烷-1-羧酸（ACC）供应给健康叶片时，它在受伤后促进乙烯释放，并抑制从这些叶片分离的条中的气孔开放。细胞分裂素玉米素和激动素（1×10⁻⁵mol m⁻³）也促进了来自感染叶片健康和生锈区域的条中的气孔开放，但只有壳梭孢菌素（1×10⁻⁶mol m⁻³）促进生锈区域的气孔大开。有人提出，内源性乙烯是蚕豆感染叶片健康或生锈区域制备的表皮条中气孔行为的有效调节剂。
