MSU-DOE Plant Research Laboratory, Michigan State University, 48824, East Lansing, MI, USA.
Planta. 1979 Oct;146(5):649-56. doi: 10.1007/BF00388846.
1-Aminocyclopropane-1-carboxylic acid (ACC) stimulated the production of ethylene in subapical stem sections of etiolated pea (cv. Alaska) seedlings in the presence and absence of indole-3-acetic acid (IAA). No lag period was evident following application of ACC, and the response was saturated at a concentration of 1 mM ACC. Levels of endogenous ACC paralleled the increase in ethylene production in sections treated with different concentrations of IAA and with selenoethionine or selenomethionine plus IAA. The IAA-induced formation of both ACC and ethylene was blocked by the rhizobitoxine analog aminoethoxyvinylglycine (AVG). Labelling studies with L-[U-(14)C]methionine showed an increase in the labelling of ethylene and ACC after treatment with IAA. IAA had no specific effect on the incorporation of label into S-methylmethionine or homoserine. The specific radioactivity of ethylene was similar to the specific radioactivity of carbon atoms 2 and 3 of ACC after treatment with IAA, indicating that all of the ethylene was derived from ACC. The activity of the ACC-forming enzyme was higher in sections incubated with IAA than in sections incubated with water alone. These results support the hypothesis that ACC is the in-vivo precursor of ethylene in etiolated pea tissue and that IAA stimulates ethylene production by increasing the activity of the ACC-forming enzyme.
1-氨基环丙烷-1-羧酸（ACC）在存在和不存在吲哚-3-乙酸（IAA）的情况下，刺激黄化豌豆（cv. Alaska）幼苗亚顶茎段中乙烯的产生。施用 ACC 后没有明显的潜伏期，并且在 1 mM ACC 的浓度下反应达到饱和。内源 ACC 的水平与用不同浓度的 IAA 以及硒代乙硫氨酸或硒代蛋氨酸加 IAA 处理的部分中乙烯产量的增加平行。根瘤菌素类似物氨基乙氧基乙烯基甘氨酸（AVG）阻断了 IAA 诱导的 ACC 和乙烯的形成。用 L-[U-(14)C]蛋氨酸进行的标记研究表明，用 IAA 处理后，乙烯和 ACC 的标记增加。IAA 对 S-甲基蛋氨酸或高丝氨酸掺入标记没有特异性影响。用 IAA 处理后，乙烯的比放射性与 ACC 的碳原子 2 和 3 的比放射性相似，表明所有的乙烯都来自 ACC。与仅用水分孵育的部分相比，用 IAA 孵育的部分中形成 ACC 的酶的活性更高。这些结果支持了 ACC 是黄化豌豆组织中乙烯的体内前体的假设，并且 IAA 通过增加形成 ACC 的酶的活性来刺激乙烯的产生。
